Recombinant PD-1 antibody (AA 32-160)

Catalog No. ABIN7543294

This anti-PD-1 antibody is a Rabbit Chimeric antibody detecting PD-1 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for Recombinant PD-1 antibody (AA 32-160) (ABIN7543294)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This PD-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 11F5C5

Product Details anti-PD-1 Antibody

Binding Specificity: AA 32-160

Purpose: Anti-PD-1 [11F5C5], Rabbit IgG, kappa

Specificity: This antibody binds the extracellular domain of the human PD-1. PD-1 is an immunosuppressive receptor having a molecular weight of 50 to 55 kD, a type 1 transmembrane glycoprotein belonging to immunoglobulin CD28 / CTLA-4 family members. It is an inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. It delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant human PD-1 protein comprising amino acids 32-160.

Isotype: IgG kappa

Application Details

Application Notes: This antibody is highly similar to the commercially available anti-PD-1 antibody NAT105. The binding specificity of this antibody towards human PD-1 was determined using ELISA. The original IgG2a antibody binds human PD_1 with an affinity constant of 7.13X10^9 M^(-1). This antibody is capable of detecting human PD-1 in a western blot assay. This antibody was used in the immunohistochemical staining of samples of T cell lymphomas and B cell lymphomas (CN109293775).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PD-1

Background: CD279, PD1, PD 1, hPD-1, PDCD1, Programmed cell death protein 1, Programmed cell death 1, Protein PD-1, CGMCC NO.16209, NAT105

UniProt: Q15116

Pathways: Cancer Immune Checkpoints